New - ExosuperQC® MSC-Specific Protein Exosome WB Characterization Kit - CD13 & CD73

Identification of mesenchymal stem cell-derived exosomes (MSC-EVs) requires dual validation of "EVs universal markers" and "MSC-specific proteins".

Quick Facts

Exosome Detection & Identification
Products
general description Identification of mesenchymal stem cell-derived exosomes (MSC-EVs) requires dual validation of "EVs universal markers" and "MSC-specific proteins".CD13 and CD73 are highly expressed characteristic proteins on the surface of MSC membranes,…

Overview

general description

Identification of mesenchymal stem cell-derived exosomes (MSC-EVs) requires dual validation of "EVs universal markers" and "MSC-specific proteins".CD13 and CD73 are highly expressed characteristic proteins on the surface of MSC membranes, are highly enriched in MSC-EVs, and can be used as ancillary markers for the traceability of MSC-EVs. The ExosuperQC ® Western Blot Kit for Characterization of MSC-Specific Proteins in Extracellular Vesicle provides highly specific rabbit-derived CD13, CD73 primary and secondary antibodies validated by EVs samples; and a built-in human umbilical cord mesenchymal stem cell (HUMSC) lysate as a positive control, which can be used to monitor the reliability of experimental operations and WB processes. This kit is designed for human stem cell-derived EVs such as HUMSC to help researchers quickly and standardize the WB traceability identification and sample quality control of CD13 and CD73 in MSC-EVs.

Specifications

general description

Specifications

Components

1. ExosuperQC ® Stem Cell Specific Protein exosome WB Identification Kit (Package) Components:

Strength

1. High antibody quality: 1. The antibodies are all rabbit-derived, and the cost is high, but they have the advantages of high affinity with the antigen and more stable signal. 2. Complete product types/free combinations: stem cell-specific protein exosome WB identification kit (cost-effective), and individual antibodies can also be purchased. These antibodies use the same reducing loading buffer and secondary antibody, which can be freely combined. 3. Complementary secondary antibodies: Secondary antibodies have also been validated by a variety of exosome samples, without considering issues such as secondary antibody selection and dilution ratio. 4. Give HUMSC cell lysate CL: Do WB with the sample, which can be used to troubleshoot any problems in the operation process.

Sample

1. Use Enze ContrExo ® high-concentration HUMSC exosomes (Cat # CTE-052), high-purity HUMSC exosomes (Cat # CTE-05), 293F exosome reference (Cat # CTE-06) as non-MSC exosome control, and the positive control CL in this kit. Use the antibody of this kit (Cat # EQC-SAP-01) for WB identification. The results show that the CD13 and CD73 antibodies in this kit have MSC exosome specificity, as follows:

Manual Downloads

Documents

EQC-SAP-01-ExosuperQC-MSC-WB-Kit-User-Manual-EN.pdf

EQC-SA1-01-ExosuperQC-MSC-WB-Kit-User-Manual-EN.pdf

EQC-SA2-01-ExosuperQC-MSC-WB-Kit-User-Manual-EN.pdf